October 29, 2018
CFI S Plan Fluor LWD 20XC and CFI S Plan Fluor LWD ADM 20XC for biological microscopes
Nikon Corporation (Nikon) is pleased to announce the release of the CFI S Plan Fluor LWD 20XC and CFI S Plan Fluor LWD ADM 20XC objectives for biological microscopes. These objectives are effective in research utilizing stem cells and drug design research. They are capable of a long working distance*1 and provide a high numerical aperture. These objectives provide clear images even in observation with plastic cell-culture dishes, which are frequently used in various types of research.
The CFI S Plan Fluor LWD 20XC is suitable for brightfield, fluorescence and DIC observation, and the CFI S Plan Fluor LWD ADM 20XC can be used for brightfield, fluorescence and phase contrast observation.
*1 Working distance: The distance from the tip of the objective lens to the observation target when the target is in focus. A long working distance has numerous advantages, such as prevention of contact with the sample when changing objectives.
Product Information
| Product Name | CFI S Plan Fluor LWD 20XC objective for biological microscopes
CFI S Plan Fluor LWD ADM 20XC objective for biological microscopes |
| Availability | October 31, 2018 |
Product Concept
Plastic cell-culture dishes are usually used in research utilizing stem cells because they enable stable sample cultivation. Compound evaluations and toxicity tests using large numbers of stem cells are conducted in drug design development. In these fields of research, there is also a demand for effective acquisition of sharp images using plastic dishes.
To meet this demand, Nikon has been developing the CFI S Plan Fluor LWD 20XC and CFI S Plan Fluor LWD ADM 20XC objectives to provide both a long working distance and a high numerical aperture (NA). They enable rapid acquisition of bright, high-resolution images, improving cell screening throughput.
Main Features
1. Long working distances and high NA
The new objectives provide long working distances of 1.3 mm to 2.3 mm, which are compatible with observations using thick-bottomed plastic dishes and observations of thick observation targets. In addition, the high NA of 0.70 enables rapid acquisition of sharp images even during fluorescence observation, which requires bright images.
2. 25 mm FOV supports wide visual field observation
The new objectives provide 20X observations with an FOV of 25 mm*2 when used together with the ECLIPSE Ti2 Inverted Research Microscope. They allow effective observation of wide areas and rapid acquisition of large amounts of observation data, enabling high cell screening and image stitching*3 throughput.
*2 FOV (field of view: the diameter of the observable image in a microscope)
*3 Image stitching: an image processing method that combines neighboring images to create one large image.
Specifications
| Product name | CFI S Plan Fluor LWD 20XC
CFI S Plan Fluor LWD ADM 20XC |
| Magnification | 20X |
| Numerical aperture (NA) | 0.70 |
| Working distance (WD) | 1.3-2.3 mm |
| Parfocal distance | 60 mm |
| Compatible plastic thicknesses | 0 ~ 1.8 mm |
| Compatible cover glass thicknesses | 0 ~ 0.17 mm |
| Compatible observation methods | Brightfield, darkfield, fluorescence, DIC*1, phase contrast*2, simple phase contrast*1
*1 Only with the CFI S Plan Fluor LWD 20XC *2 Only with the CFI S Plan Fluor LWD ADM 20XC |
Sample Images
Fluorescence images of the actin, nuclei and mitochondria of HeLa cells are simultaneously acquired and then overlaid. Acquired with the CFI S Plan Fluor LWD 20XC objective mounted on the Confocal Microscope A1+.
An overall image of HeLa cells (acquired by phase contrast observation) and an image of nuclei and mitochondria (simultaneously acquired by fluorescence observation) are overlaid. Acquired with the CFI S Plan Fluor LWD ADM 20XC objective mounted on the Inverted Research Microscope Ti2-E.