Capabilities of the Lexogen RNA-Seq library preparation protocols have been expanded with the launch of 96 i5 x 96 i7 Dual Indexing Barcodes and Unique Molecular Identifiers.
We are happy to announce the launch of two new products that complement the Lexogen SENSE and QuantSeq RNA-Seq library preparation protocols.
96 i5 x 96 i7 Dual Indexing Barcodes
Unleash the power of your RNA-Seq experiments with 9,216 unique dual indexing barcodes
- 96 i5 x 96 i7 unique barcode combinations for fully-flexible dual indexing
- 6 bp indices with perfect, uniform nucleotide and color balance
- Hamming distance of 3 enables both error detection and error correction
- Compatible with sequencing on all Illumina instruments
- Detect and quantify index hopping
- Avoid cross-contamination and minimize index mis-assignment
- Online index balance checker tool will be shortly available to assist with the choice of the optimal index combination for your experiment
- Use with all QuantSeq 3’ mRNA-Seq and SENSE RNA-Seq Library Prep Kits for Illumina
Check the Instruction Manual for the i5 Unique Dual Indexing Add-on Kit for QuantSeq/SENSE (5001-5096) for more details.
Find the i5 Unique Dual Indexing Add-on Kits for QuantSeq/SENSE (5001-5096) in the Lexogen web store.
Unique Molecular Identifiers (UMIs)
Analyze and de-duplicate your sequencing data for unbiased gene expression profiling
- Tag individual transcripts to identify PCR duplicates
- Detect and eliminate amplification bias
- Use with QuantSeq 3’ mRNA-Seq Library Prep Kit (FWD) for Illumina
- Simply exchange one solution in the standard QuantSeq FWD protocol
- Use for single and dual-indexed libraries
Check the Instruction Manual for the UMI Second Strand Synthesis Module for QuantSeq FWD for more details.
Find the UMI Second Strand Synthesis Module for QuantSeq FWD in the Lexogen web store.