January 19, 2018
For LabSolutions LCMS
Mycotoxins are highly toxic chemical byproducts from fungi commonly found in crops, and residual content in foods are regulated the world over. This method package provides simultaneous multi-component analysis conditions for mycotoxins subject to the regulations in Japan, the USA, the EU, and China.
Analytical Methods for 27 Mycotoxins
This package contains seven different analytical method conditions and fully optimized MRM transitions for a total of 27 different mycotoxins. Rapid analytical methods are included for a 25 mycotoxin screen, 16 mycotoxins using high-sensitivity conditions, 10 mycotoxins using APCI, and specialized methods for trichothecenes, fumonisins, aflatoxins, and patulin.
Analytical Method for 25 Mycotoxins in 15 Minutes
HPLC Conditions
Column ： Mastro™ PFP2 (Shimadzu GLC)
(2.1 mmI.D. x 150 mmL, 3 µm)
Mobile Phase A ： 10 mmol/L aqueous ammonium acetate
Mobile Phase B ： Methanol (2 % acetic acid)
Flowrate ： 0.4 mL/min
Column Temp. ： 40 °C
MS Conditions
Ionization Mode ： ESI（+/-）
Nebulizer Gas Flowrate ： 2 L/min
Drying Gas Flowrate ： 10 L/min
Heating Gas Flowrate ： 10 L/min
Interface Temp. ： 300 °C
DL Temp. ： 250 °C
Heat Block Temp. ： 500 °C
High-Speed Simultaneous Analysis of 25 Mycotoxins
Compound List for Each Method
| Method name | Detail | Analysis time | |
|---|---|---|---|
| A | A_MRM_25_PFP_15m | Simultaneous analysis of 25 mycotoxins | 15 min |
| B | B_MRM_16_ODS_30m | Simultaneous analysis with high sensitivity | 30 min |
| C | C_MRM_1_PAT_ODS_10m | Patulin analysis | 10 min |
| D | D_MRM_6_AF_ODS_30m | Aflatoxins analysis | 30 min |
| E | E_MRM_4_DONNIV_ODS_12m | Trichothecenes analysis | 12 min |
| F | F_MRM_3_FB_ODS_15m | Fumonisins analysis | 15 min |
| G | G_MRM_10_ODS_33m | 10 mycotoxins using APCI | 33 min |
AF B1: aflatoxin B1 AF B2: aflatoxin B2 AF G1: aflatoxin G1 AF G2: aflatoxin G2 AF M1: aflatoxin M1
AF M2: aflatoxin M2 DON: deoxynivalenol 3ADON: 3-acetyl-deoxynivalenol 15ADON: 15-acetyl-deoxynivalenol
NIV: nivalenol FUX: 4-acetyl-nivalenol FB1: fumonisin B1 FB2: fumonisin B2 FB3: fumonisin B3 OTA: ochratoxin A
PAT: patulin T-2: T-2 toxin HT-2: HT-2 toxin ZEN: zearalenone α-ZEL: α-zearalenol β-ZEL: β-zearalenol
α-ZAL: α-zearalanol β-ZAL: β-zearalanol DAS: diacetoxy-scirpenol NEO: neosolaniol D3G: deoxynivalenol-3-glucoside
STE: sterigmatocystin
* Mastro is a trademark of SHIMADZU GLC Ltd.