Thu 21st May 2020
Latest kit release provides 96 native barcodes for PCR-free sequencing, allowing higher numbers of samples to be run on a single flow cell – including for SARS-CoV-2 whole genome sequencing protocol.
The Native Barcoding Expansion (96) kit is available to purchase on the Store now here.
Oxford Nanopore provides direct sequencing of a nucleic acid molecule – either DNA or RNA – examining the native molecule instead of a copy, or via surrogate markers such as fluorescence. This direct sequencing avoids introducing biases, and leaves base modifications intact, giving even genome coverage. With native barcodes this PCR-free sequencing is preserved, allowing up to 96 samples to be sequenced at once on a single flow cell. Multiplexing more samples significantly lowers the cost per individual sample.
![](https://www.scientifictechnologynews.com/wp-content/uploads/2021/05/image-1024x816.png)
Most recently the Native Barcoding Expansion kits have been used in the protocol for whole genome sequencing of SARS-CoV-2, the virus that causes COVID-19. Performing genomic epidemiological analysis of the transmission and evolution of the SARS-CoV-2 virus has become a key part of the global public health response to the COVID-19 pandemic. To support this effort, earlier in the year a protocol was released for rapid, scalable and cost-effective whole genome sequencing of the virus using nanopore technology.
With the release of 96 native barcodes, this assay can now scale from 1-96 samples per run, and across our range of devices to enable up to approximately 4,500 samples per run.
View the protocol https://community.nanoporetech.com/protocols/pcr-tiling-ncov/v/PTC_9096…“>here.